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. 2011 Jun 2;7(6):e1002106. doi: 10.1371/journal.pgen.1002106

Figure 6. Activation and inactivation of CorE.

Figure 6

(A) CorE is not degraded. M. xanthus cells harboring the hCorE protein were harvested at the times (h) indicated above each lane after the addition of 0.3 mM copper sulfate and analyzed by Western blot using an anti-His tag antibody. (B) cuoB is not up-regulated by any of the additives indicated in the panel. (C) CorE is activated and inactivated by the redox state of copper. cuoB expression was analyzed in the presence of different additives that modify the redox state of copper, that mimic Cu(I), or that chelate copper in any of its two redox states. For panel B and C, M. xanthus cells harboring the fusion cuoB-lacZ were incubated on CTT agar plates containing the additives indicated. Samples were harvested at different times and β-gal specific activity was determined. Error bars indicate standard deviations.