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. 2011 Jun 2;6(6):e18691. doi: 10.1371/journal.pone.0018691

Figure 5. Inhibition of autophagy enhances the cytotoxity of EGFR-TKIs in lung cancer cells.

Figure 5

A, Autophagy was induced by EGFR-TKIs in resistant but not sensitive lung cancer cells. LC3 expression in lung cancer cells incubated with 25 µM gefitinib or erlotinib for 24 h were analyzed by immunoblotting. B, The viability of cancer cells treated with 12.5 µM gefitinib or erlotinib for 48 h with or without 5 µM CQ were measured by MTT assay. C, The expression of ATG5 and ATG7 in A549 cells transiently transfected with negative control siRNA, Atg7 or Atg5 siRNA were determined by immunoblotting. D, Cells transiently transfected with negative control siRNA, Atg7 or Atg5 siRNA were treated with 12.5 µM gefitinib or 25 µM erlotinib for 48 h. The effect of EGFR-TKIs on cancer cells with or without ATG5 or ATG7 knockdown were analyzed by MTT assay. NC, Control siRNA. Data were expressed as the mean ± SD, and analyzed by Student's t test. * P<0.05, ** P<0.01.