Figure 7. IL-10 is required for IVIG's anti-inflammatory effects in the CNS.

(A) Quantification of IFN-γ, TGF-β and IL-10 mRNA at d6 pi in BS of infected 129 mice treated with PBS or IVIG. (B) Intracellular IFN-γ and IL-10 expression after antigen stimulation of CD45^high (top) or CD4⁺ T cells (bottom) at d6 and d14 pi in the BS of infected 129 mice given either IVIG or PBS. (C) BS CD4⁺ T cells in IVIG treated (top) or control (bottom) 129 mice at d6 pi analyzed for ICOS and IL-10 expression with (right) or without (left) PMA + ionomycin; plots gated on CD45^high CD4⁺ T cells. (D) HSV infected 129 WT or IL-10 KO mice were given IVIG or PBS at 24 h pi and monitored for survival. (E) FACS plots gated on CLN derived CD4⁺ T cells showing CD25 and ICOS expression. (F) BS CD45^high infiltrates in IVIG treated HSV infected IL-10 KO mice at d8 pi analyzed for expression of MHC II and CD11b (top). FACS plots gated on BS CD45^high F480⁺ macrophages depicting Ly6C and MHC II expression (bottom). (G) Degranulation of CD45^high gated cells as measured by CD107a/b expression (blue dots) in the absence of HK-HSV stimulation in the brain (top) and BS (bottom) of PBS (left) or IVIG (right) treated (right) infected IL-10 KO mice at d 8 pi. Isotype control shown in red. Data representative of 2–4 experiments (n = 6–12 mice).