Figure 8. Cloning of Gamma, Mu, Kappa and Lambda immunoglobulin fragments by blunt-end and QC cloning.

(A) PCR products amplified from G-tailed cDNA prepared from non-Hodgkin lymphoma biopsy sample T019 (isotype Gamma, Lambda) and T069 (isotype Mu, Kappa). Amplification was performed using primer bap2 pc and primers GC3F, LC1N, Mu1F and KC2F as indicated. The PCR products were cloned using QC cloning (B and D) or blunt-end cloning (C and E). 12 randomly chosen clones for each reaction were analyzed by colony PCR using vector primers. The expected size of full-length inserts is indicated by a dashed line and an arrow.