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. 2011 Jun 2;7(6):e1002073. doi: 10.1371/journal.ppat.1002073

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Tekes et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Schematic of the VSV genome containing an (A-) leader region and a 60-nt long gene inserted between the leader region and the N gene. The complete sequence of the leader region is shown. The positions at which G residues were engineered to replace the original A residues in the gene-start sequence are underlined (B) In vitro transcription reactions using 10 µg of each of the purified viruses were performed in the presence of [α-³²P]-GTP. Purified RNA was analyzed on acid-agarose gel and detected using a phosphorimager. The identity of the mRNAs is shown on the right.