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. 2011 Jun 2;6(6):e20549. doi: 10.1371/journal.pone.0020549

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Lim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Combined chemical shift change plots of apo-meACP versus (A) holo-meACP, (B) hydroxybutyryl-meACP and (C) octanoyl-meACP. Combined chemical shift change plots of (D) hydroxybutyryl-meACP versus holo-meACP, (E) octanoyl-meACP versus holo-meACP and (F) octanoyl-meACP versus hydroxybutyryl-meACP. A combined chemical shift cut-off of 0.05 ppm is shown as the dashed line. The residue number and the positions of the helices (α1, α2 and α3) are also indicated. (G) Ribbon representation of apo-meACP with the positions of the perturbed residues upon phosphopantetheinylation are highlighed (combined chemical shift cut-off >0.05 ppm (yellow sphere) and >0.10 ppm (red sphere)). (H) Ribbon representation of apo-meACP with the positions of the perturbed residues based on (F) upon acylation by octanoyl moiety are highlighted (combined chemical shift cut-off >0.05 ppm (blue sphere)).