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. 2011 Jun 2;6(6):e20598. doi: 10.1371/journal.pone.0020598

Figure 7. Sin/IL12 induces the NK cell-dependent, IFNγ-dependent expression of MHC class II on peritoneal macrophages.

Figure 7

(A) ES-2 bearing mice were treated for one week (4 treatments) with media, Sin/LacZ, Sin/LacZ + anti-CD122 (NK cell depleted), Sin/IL12, or Sin/IL12 + anti-CD122, after which mice were euthanized, and the expression of MHC class II on peritoneal macrophages (F4/80+ FSC high cells) was analyzed by FACS (left panel), and quantified (right panel) (n = 2–3 as shown). Black lines represent NK cells from the various Sindbis-treated mice, and the solid grey curve represents NK cells from mock-treated mice. (B) ES-2 bearing mice were treated daily for two days with media, Sin/LacZ, Sin/IL12, Sin/IL12 + anti-IFNγ (IFNγ block), or Sin/IL12 + anti-CD122 (NK cell depleted). MHC expression was analyzed and quantified as in A. Data is from one experiment (n = 4).