Figure 1. Receptor variants can protect J774A.1 cells from LeTx intoxication.
A. vWA domains of anthrax toxin receptors/variants were fused with N-terminal His tags, purified by Ni affinity chromatography and anion exchange chromatography and analyzed with SDS-PAGE. B. The results for cell protection assays. Viability was assessed as described in the methods section. Each assay was performed at least three times, with duplicates within each assay. Data points represent the mean ± SEM in duplicate for one representative experiment. C. The results for the Schild plot assays; a, b, and c. PA dose-dependent survival curves for cells at a fixed receptor/mutant concentration; d, e, and f. These graphs were obtained by plotting the EC50 values corresponding to different receptor concentrations, fixed by linear regression using GraphPad Prism software. Each assay was performed at least three times. The data shown are for one representative experiment. a Samples were not mixed with 2-mercaptoethanol.