Table 3.
Comparison of the Characteristics of Mu and Ty1 Sequencing Strategies
| Mu | Ty1 | |
|---|---|---|
| Reaction catalyzed by | MuA transposase | Ty1 virus-like particles |
| Donor DNA length (kb) | 1.2 | 0.9 |
| Introduction of reaction products into cells | transformation/electroporation | electroporation |
| Reaction efficiency | ||
| maximum reported (insertions per target) | 1/10 | 1/2.5 × 103 |
| Target site duplication (bp) | 5a | 5b |
| Standard reaction | ||
| donor used (ng) | 18 | 100–500 |
| target used (ng) | 500 | 1000 |
| molar ratio of transposon to target | 1:2 | 10:1 |
| Reported recovery of clones | ||
| minipreps studied (no., %) | 95, 100 | 153, 100c |
| double insertions/cotransformants (%) | 2 | 8 |
| unknown plasmid map (%) | 0 | 3 |
| no transposon (%) | 0 | <1 |
| single insertion in one plasmid (%) | 98 | 88 |
Mu characteristics (this study); Ty1 characteristics (Devine and Boeke 1994).
a
Allet (1979); Kahmann and Kamp (1979); Mizuuchi and Mizuuchi (1993); this study.
b
Eichinger and Boeke (1988, 1990); Ji et al. (1993); Braiterman and Boeke (1994); Primer Island Transposition Kit Protocol (Applied Biosystems).
c
The numbers for the Ty1 system were calculated from Table 2 of Devine and Boeke (1994). For comparison the number of minipreps studied was adjusted to 100%