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. 2011 May 12;108(22):9160–9165. doi: 10.1073/pnas.1014235108

Fig. 5.

Fig. 5.

Posterior somite budding is not dependent on FGF signaling. Expression of MRF1 at the late neurula stage in control embryos (A) and SU5402-treated embryos (treatment 4) (A′). (Scale bars = 50 μm.) Expression of Brachyury2 in control embryos (B) and in embryos fixed at the larva stage after treatment 4 with SU5402 (B′). In the control embryos, Brachyury2 is expressed in the most anterior notochord and in the tailbud, whereas in the treated embryos, Brachyury2 is still expressed in the posterior notochord anterior to the tailbud. (Scale bars = 50 μm.) (C and C′) F-Actin staining of larval posterior striated muscle fibers in control embryos and in embryos after treatment 4 with SU5402. (Scale bars = 50 μm.) TEM pictures of transverse sections at the larva stage of the posterior region of control embryos (D) and of embryos after treatment 4 with SU5402 (D′). Arrows indicate the space between the somitic cells and the epidermis.(Scale bars = 10 μm.) (E) Schematic representation of the different somitic regions in amphioxus. Dorsal view; anterior is to the left.