Table 1.

Kinetic parameters of apoMb transient unfolding and refolding measured by R₂ relaxation dispersion

Probes	pH	kN-I1 , s-1	kI1-N , s-1	kI1-MG, s-1	kMG-I1, s-1	PN	PI1	PMG
*	5.50	38 ± 1	495 ± 7	—	—	0.93 ± 0.02	0.07 ± 0.00	—
†	4.95	31.7 ± 0.5	240 ± 12	53 ± 1	26 ± 4	0.71 ± 0.03	0.09 ± 0.00	0.20 ± 0.03
3CO/15N‡	4.75	78 ± 18	650 ± 140	113 ± 16	17 ± 1	0.52 ± 0.01	0.06 ± 0.01	0.42 ± 0.01

^*Parameters from a two-state fit to amide datasets ¹H-SQ and ¹⁵N-SQ using the cluster of residues in contact with the F helix and the first helical turn of G (listed in the caption of Fig. S2). Error values were obtained using Monte Carlo sampling.

^†Parameters from a three-state fit (Scheme 1) to the data shown in Fig. 2 (¹H-SQ, ¹⁵N-SQ, ¹H¹⁵N-DQ, ¹H¹⁵N-ZQ). Error values were obtained using Monte Carlo sampling.

^‡Parameters from a three-state global fit (Scheme 1) to data shown in Fig. 3. Error values were derived from an extensive grid search of the kinetic parameter space (SI Text).