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. 2011 May 5;108(22):E149-E158. doi: 10.1073/pnas.1102223108

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Fig. 3. — Characterization H3K27me3 localization and its role in myogenesis (A, B) Patterns of H3K27me3 and its anticorrelation with H3K36me3 across a region of chromosome 10 (Left) and the Myh loci (Right). The y-axis shows the log ₂ of the enrichment. (C) ChIP-seq localization of H3K27me3, p300, H3K4me1/3, and Pol II on Myog. Blue bars at the bottom indicate fragments tested for enhancer activity. The y-axis shows the log ₂ of the enrichment. (D) Luciferase assay testing the enhancer activity of fragments up- and downstream of Myog coding region. (E) Analysis of extracts and chromatin after miRNA-mediated Suz12 depletion in myoblasts and myotubes showing effect on indicated proteins and global H3K27me3 levels by Western blotting (Left) and recruitment to chromatin by ChIP (Right). (F) Quantitative chromatin IP (qChIP) showing the effect of Suz12 depletion on H3K37me3 levels at Myog genomic regions shown in panel C.