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Inhibition of autoprocessing of protease precursors derived from natural variants, M (TFR-PRM), N (TFR-PRN), and P (TFR-PRP), by DRV and SQV in E. coli and analyses by SDS/PAGE. Letters a, b, and c correspond to the full-length precursor, intermediate precursor, and the mature protease, respectively, as shown in the schematic in Fig. S1A for TFR-PRM. M denotes standards in kDa.
