Fig. 6.
Comparison of the thermal stabilities of the precursor (TFR-PRM) and the mature protease (PRM) complexed to PIs and PRM (dashed black) in the absence of PI. Data for PRM (Top) are from (5, 13) for comparison. DSC scans were carried out using approximately 14 μM PRM and 8–10 μM TFR-PRM (as dimers) in the presence of approximately twofold molar excess of DRV, SQV, or ATV. Tm values (°C) are shown in parentheses. For TFR-PRM bound to DRV, SQV, and ATV, values of Tm.diff (Tm of mature PR-PI complex minus Tm of TFR-PRPI complex) are 20.2, 27.0, and 27.3 °C, respectively. Dashed gray lines depict the deconvolution of the two apparent transitions discussed in ref. 5.