Fig. 3.

Direct regulation of HyWnt3act by Tcf. (A) EMSA shows binding of recombinant His-tagged XTcf-3 protein to the Tcf sites 1, 2, and 3. (B) Schematic diagram of the HyWnt3act element with the location of TCF-binding sites indicated. Boxes and X (red) indicate WT and mutant sites, respectively. (C–F) Requirement of the Tcf sites in Hydra in vivo. Mutation of all Tcf sites (C) abolished reporter activity. Tcf sites 2 (E) and 3 (F), but not 1 (D), were essential for the activity. (Scale bar: 100 μm.)