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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Yeast. 2011 Mar 30;28(6):495–503. doi: 10.1002/yea.1853

Figure 4.

Figure 4

arc3 function has been conserved throughout evolution. (A) HeLa cells expressing human ARPC3-EGFP were fixed and stained with rhodamine-phalloidin to visualize F-actin. We found that nearly every GFP dot overlaps with an F-actin dot. (B) HeLa cells expressing ARPC3-EGFP were treated with 10 µM Latrunculin A for 30 min to depolymerize F-actin. These cells were then washed and incubated in regular media for 1 hr before being photographed. (C) Diploid cells heterozygous for arc3 deletion (+/arc3::ura4) were transformed with plasmids expressing S. pombe arc3 (pREP41ARC3), human ARPC3 (pREP1ARPC3) or the empty vector as indicated. Cells were then sporulated, and plated on media with selection for the arc3 deletion. Relative survival compared to control was quantified (right). (D) Colonies from cells transformed with S. pombe arc3 (pREP41ARC3) or human ARPC3 (pREP1ARPC3) obtained as in (C) were grown on MM to log phase and observed under the microscope. The arrowheads show cells with abnormal morphology and the arrow indicates a cell with an improperly positioned septum.