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Alu PCR analysis of YACs containing the targeted chromosome region. Nine isolates of circular Trp+ YACs and three isolates of Trp+ His+ linear YACs were characterized. Total yeast DNA was purified from each isolate and PCR amplified by Alu-specific primers. (Lanes 1–9) Trp+ YACs; (lanes 10–12) Trp+ His+ YACs. The last lane corresponds to a 1-kb ladder.
