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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Cancer Res. 2011 Apr 12;71(11):3904–3911. doi: 10.1158/0008-5472.CAN-11-0396

Figure 1.

Figure 1

Nrf2 enhances ABP-induced DNA damage in bladder tissues in vivo but inhibits such damage in bladder cells in vitro. (A) Nrf2+/+ mice and Nrf2−/− mice were treated with a single intraperitoneal injection of ABP or vehicle; the bladders were harvested 24 h later for determination of dG-C8-ABP levels by LC/MS/MS. (B) RT4 cells were treated with control siRNA and Nrf2 siRNA for 48 h, followed by Western blotting of Nrf2 in whole cell lysates. GAPDH is a loading control. (C) After 48 h treatment with control siRNA and Nrf2 siRNA, RT4 cells were exposed to ABP (plus S9) or N-OH-AABP for 3 h before dG-C8-ABP measurement.