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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cancer Res. 2011 Apr 11;71(11):3896–3903. doi: 10.1158/0008-5472.CAN-10-4167

Figure 2.

Figure 2

Functional Analysis of the LIN28B rs12194974 (-727 G/A) Polymorphism in vitro. A. Schematic representation of the LIN28B luciferase Reporter which contains an 1.4-kb promoter and 3 exons of LIN28B following IRES and luciferase gene. B. LIN28B SNP Promoter Activity in Ovarian Cancer Cell Lines. The -727-G and -727-A allele constructs were transfected into OV420, OV432, CAOV3, SKOV3, OV8, A2780CP, and A2780S ovarian adenonocarcinoma cell lines and T80 human immortalized ovarian surface epithelial cell line (HIOSE). β-galactosidase was used to standardize transfection efficiency. Relative luciferase activity (RLA) was measured. RLA units are presented as means, and the T bars represent standard deviations (SD). Mean ± SD from triplicates. Statistical analysis was conducted using Student’s t test. C. LIN28B SNP mRNA Expression in Ovarian Cancer Cell Lines and Immortalized Cell Line. The -727-G and -727-A allele constructs were transfected into A2780CP and A2780S and the T80 human cells. LIN28B SNP mRNA expression was determined by real-time PCR, and normalized with β-actin.