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. 2011 Jan 31;30(22):2547–2557. doi: 10.1038/onc.2010.626

Figure 3.

Figure 3

BEZ235 increases HER2/HER3 heterodimerization and receptor signaling. (a) Immunoblots of lysates from BT474 and MCF7-HER2 cells treated with DMSO (c) or BEZ235 (b, 500 n) for 24 h and subjected to membrane cross-link (Xlink). Cross-linked protein lysates were immunoprecipitated with (IP) and immunoblotted (IB) against the indicated antibodies. Whole-cell lysate (WCL) serve as input controls. (b) Immunoblots of lysates from MCF7-HER2 cells treated with BEZ235 (b, 0–500 n), lapatinib (L, 500 n) or the combination (B+L, both at 500 n) for 24 h. Total lysates were immunoprecipitated with (IP) and immunoblotted (IB) against the indicated antibodies.