Figure 5.
Ara h 2 Epitope Data and Fractional Side Chain Accessibility. Black bars plot the fractional side chain accessibility determined by VADAR (38). Overlayed on the plot, residues that are inaccessible due to MBP measured with a 1.4, 10, and 17 Å sphere are highlighted red, orange, and yellow, respectively. Other residues are highlighted green. Other highlights indicate: disordered residues and estimated side chain accessibility (grey); Helices (cyan); potentially important peptide IgE epitopes in (5) (magenta); T-cell epitopes (37) (black box). Arrows indicate the positions of a multiple alanine-substituted mutant of Ara h 2 with reduced IgE binding (12). Dotted lines indicate disulfide bonds.