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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Vet J. 2011 Feb 10;191(2):231–239. doi: 10.1016/j.tvjl.2010.12.030

Table 1.

Real time RT-PCR primer sequences.

Lineage Gene Primer sequence
Housekeeping GAPDH 5′-TGG CAA AGT GGA TAT TGT CG
3′-AGA TGG ACT TCC CGT TGA TG
Adipogenic PPAR-γ 5′-TTC TCC AGC ATT TCC ACT CC
3′-AGG CTC CAC TTT GAT TGC AC
Leptin 5′-TGT GTT GAA GCT GTG CCA AT
3′-CCC TCT GTT TGG AGG AGA CA
Osteogenic COL1α1 5′-GTG TGT ACA GAA CGG CCT CA
3′-TCG CAA ATC ACG TCA TCG
OPG 5′-TGT CTA TAC TGC AGG CCG GTG
3′-TCA GGC AGA ACT CAA GCT CCA

PPAR-γ, peroxisome proliferator-activated receptor gamma; COL1α1, collagen type I alpha 1; OPG, osteoprotegerin.