Table 1.
Lineage | Gene | Primer sequence |
---|---|---|
Housekeeping | GAPDH | 5′-TGG CAA AGT GGA TAT TGT CG 3′-AGA TGG ACT TCC CGT TGA TG |
Adipogenic | PPAR-γ | 5′-TTC TCC AGC ATT TCC ACT CC 3′-AGG CTC CAC TTT GAT TGC AC |
Leptin | 5′-TGT GTT GAA GCT GTG CCA AT 3′-CCC TCT GTT TGG AGG AGA CA |
|
Osteogenic | COL1α1 | 5′-GTG TGT ACA GAA CGG CCT CA 3′-TCG CAA ATC ACG TCA TCG |
OPG | 5′-TGT CTA TAC TGC AGG CCG GTG 3′-TCA GGC AGA ACT CAA GCT CCA |
PPAR-γ, peroxisome proliferator-activated receptor gamma; COL1α1, collagen type I alpha 1; OPG, osteoprotegerin.