Effects of 6BIO on human melanoma cell lines. A, MTS cell viability assays were performed in A2058, G361, SK-MEL-5, and SK-MEL-28 human melanoma cells. Cells were treated with 6BIO at various concentrations for 48 h. B, human melanoma cells were treated with 10 μmol/L of 6BIO for 4 h. Cells were lysed for Western blot analysis using antibodies specific to p-Jak2, Jak2, p-Src, Src, p-Stat3, Stat3, and β-Actin.