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. 2011 Jan 28;23(2):149–158. doi: 10.1093/intimm/dxq465

Fig. 1.

Fig. 1.

SLE-like humoral autoimmunity in Slamf1−/− [B6.129] mice. (A) Anti-nucleosome auto-antibodies in aged Slamf1−/− [B6.129] mice but not in Slamf1−/− [BALB/c.129] mice. Anti-nucleosome auto-antibody titers in the serum of 10- to 13-month-old females were determined by ELISA, as described in Methods. B6 n = 38; Slamf1−/− [B6.129] n = 16; Slamf1−/− [BALB/c.129] n = 5; BALB/c n = 9. (B) Antinuclear auto-antibodies in Slamf1−/− [B6.129] mice. Permeabilized Hep-2 cells were incubated with sera from 1-year-old female Slamf1−/− [B6.129] (n = 7) and B6 (n = 7) mice. After washes, bound IgG was detected with anti-mouse IgG-Alexa488 (see Methods). Demonstrated staining pattern is representative for the Slamf1−/− [B6.129] group. (C) Progressive humoral autoimmunity in Slamf1−/− [B6.129]. Penetrance of anti-nucleosome IgG in aged female Slamf1−/− [B6.129] mice by age groups (±1 month). A serum was judged positive if auto-antibody titer was above mean titer of the B6 group plus 2 SD. (D) Mild splenomegaly in aged Slamf1−/− [B6.129] mice. (E) Increased percentage of effector/memory CD4+ T cells in Slamf1−/−[B6.129] mice. Determined by flow cytometry as described in Methods. Ten- to 13-month-old female B6 (n = 25) and Slamf1−/− [B6.129] (n = 10) mice were used for both panels.