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. 2011 Jan 28;23(2):149–158. doi: 10.1093/intimm/dxq465

Fig. 3.

Fig. 3.

Hapten-specific antibody responses in Slamf1−/− [BALB/c.129] and Slamf2−/− [BALB/c.129] mice. (A) Type I T-independent B-cell responses in Slamf1−/− [BALB/c.129] and Slamf2−/− [BALB/c.129] mice. BALB/c, Slamf1−/− [BALB/c.129] and Slamf2−/− [BALB/c.129] mice (n = 4) received one i.p. injection of 10 μg of TNP–LPS. TNP-specific IgM (left panel) and IgG (right panel) titers were determined at day 6 by ELISA after serial dilutions of the serum (y-axis = OD 405). Results are representative of two independent experiments. (B) Type II T-independent B-cell responses in Slamf1−/− [BALB/c.129] and Slamf2−/− [BALB/c.129] mice. BALB/c, Slamf1−/− [BALB/c.129] and Slamf2−/− [BALB/c.129] mice (n = 4) received one i.p. injection of 30 μg of TNP-Ficoll. TNP-specific IgM (left panel) and IgG (right panel) titers were determined at day 6 by ELISA after serial dilutions of the serum (y-axis = OD 405). Results are representative of two independent experiments. (C) Antibody responses to T–D antigens in Slamf1−/− [BALB/c.129] and Slamf2−/− [BALB/c.129] mice. NP-specific antibody production in the sera of BALB/c, Slamf1−/− [BALB/c.129] and Slamf2−/− [BALB/c.129] mice (n = 4) was determined 7 days after i.p. immunization with NP–KLH antigen in normal saline of mice previously immunized with alum-precipitated NP–KLH. NP-specific IgG and IgM antibody titers were determined by ELISA after serial serum dilutions (y-axis = OD 405; OD at 405 nm). NP-specific IgM antibodies were determined 10 days after primary immunization.