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Comparison of the expression levels of Smurfs between normal and scleroderma fibroblasts. Cell lysates prepared from confluent quiescent fibroblasts were subjected to immunoblotting using the indicated Ab’s. One representative of five independent experiments is shown (a). The protein levels quantitated by scanning densitometry are shown relative to those in normal fibroblasts (100 AU) (b). Data are expressed as the mean ± SD of five independent experiments.
