Skip to main content
. 2011 May 19;7(5):629–644. doi: 10.7150/ijbs.7.629

Figure 1.

Figure 1

Ceramide exposure affects cell growth and proliferation in vitro. (A) Control MCF-7 cell-line and 25-50 μM ceramide-exposed MCF-7 cell-lines treated for 24 hours. (B) Cell viability quantification illustrating the effect of ceramide on cells of the MCF-7 cell-line treated for 24 hours. Ceramide exposure altered the viability of the MCF-7 cell-lines in a concentration dependent manner. Note that at 12.5 μM ceramide, no significant cell death is induced by ceramide (black arrow). (C) Cathepsin D levels in MCF-7 cells exposed to ceramide at a concentration of 50 µM for 24 hours. Results are represented as Cathepsin D concentration (ng/mL). Data are from three experiments conducted in triplicates (* p < 0.001). (D) Westernblot of PI3K protein expression in MCF-7 cells exposed to 25 μM and 50 μM ceramide for 24 hours. (E) Visualization of monodansylcadaverine (MDC) indicative of autophagic vacuole formation in MCF-7 cells treated with ceramide at 50 μM for 24 hours.