Figure 2.

In vitro assays of tumorogenesis and tube formation, consequences of ceramide exposure. (A) Ceramide exposure inhibits cell migration. Results are expressed as percent of control (vehicle-treated cells) and the means of triplicate determinations ± SE. Data are from one experiment and are representative of three replicated experiments (*p < 0.001). (B) Effect of ceramide exposure (5 µM - 50 µM) on VEGF expression in MCF-7 cells treated for 24 hours. Results are represented as a VEGF concentration (pg/mL) after normalization based on cell viability. Data are from three experiments conducted in triplicates (p < 0.001). (C) HUVEC containing 10 ng/ml of VEGF were plated on a Matrigel and they typically aligned forming cord-like structure. The asterisks in the 25 µM example denote a region where ceramide induced tube formation has been inhibited.