Fig 2.

The effects of berberine hydrochloride on the levels of Gck (A), G6pc (B), Pck1 (C) and Srebp-1c (D) transcripts in hepatocytes from SD rat. Primary hepatocytes were treated with either vehicle or berberine hydrochloride (1 to 25 μM) for 6h in the absence or present of 1 nM insulin. The expression levels of indicated transcripts were analyzed using real-time PCR. The expression level in control group without insulin was arbitrarily assigned as 1. Results represented means ± SD of three independent experiments (* for comparing the fold induction of the indicated transcript at the berberine hydrochloride concentration in the absence with that in the presence of insulin; for Gck, b>a; for G6pc, d/e>g, c/d/e>h, d'>f'/g'/h', and e'>g'; for Pck1, n>i/j/k; for Srebp1c, t>s>o/p/q, t>r, and t'>o'/p'/q'/r'/s'; all P<0.05).