Fig 6.

Immunoblot analysis of phosphor-AMPKα, total AMPKα, phosphor-ACC, total ACC, phosphor-Akt, total Akt, and β-actin in primary hepatocyes treated with berberine hydrochloride in the absence or presence of insulin. After overnight pretreatment, primary hepatocytes from Zucker lean rats were incubated in medium A without or with increasing concentrations of berberine hydrochloride (1 to 30 µM) in the absence or presence of 1 nM insulin for 20 minutes. Total protein (30 µg/lane) was separated on SDS/PAGE, detected by specific primary antibodies as indicated, and visualized by chemiluminescence. Graph was the representative of three independent experiments with similar results using hepatocytes isolated from three different ZL rats in three different days.