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Muscle insulin signaling. Mice were treated with insulin, delivered via the inferior vena cava, and protein extracts were prepared and analyzed by immunoprecipitation with anti-phosphotyrosine antiserum followed by immunoblotting with anti-p85 antiserum (upper panel). Alternatively, extracts were resolved on SDS-PAGE and analyzed by immunoblotting with anti-pSer473-Akt antiserum (middle panel), followed by reprobing of the stripped blots with anti-Akt antiserum (bottom panel).
