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. 2004 Jan 15;113(2):265–273. doi: 10.1172/JCI18688

Figure 6.

Figure 6

Synaptic parameters recorded in hemidiaphragm neuromuscular preparations from Ncx3+/+ and Ncx3–/– mice. (a) Spontaneous quantal ACh release, measured as the frequency of MEPPs. The number of NMJs tested in each group is indicated in brackets. (b) Full-size EPP evoked at 0.1 Hz by a single nerve stimulus in a Ncx3–/– NMJ (left tracing) and after repetitive stimulation at 10 Hz (right tracing). The arrow indicates the increased MEPP frequency. Standard physiological solution supplemented with 2.2 μM μ-conotoxin GIIIB was used. The resting membrane potential during measurements was –74 ± 0.2 mV. (c) EPP amplitudes evoked at 50-Hz nerve stimulation (expressed as percent of the first EPP of each train, in sequential order). (d) Nerve-evoked, tetanus-twitch ratio at different stimulation frequencies in hemidiaphragm muscles from Ncx3+/+ and Ncx3–/– mice (n = 3). The resting membrane potential during recordings was –72 mV. Mean ± SD (a and d). *P ≤ 0.001.