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. 2011 Jan 12;120(2):381–391. doi: 10.1093/toxsci/kfq394

FIG. 3.

FIG. 3.

Activation of the 2B6-XREM-PBREM reporter by CAR1, CAR2, CAR3, and PXR after treatment with phthalates. Results shown here represent a single transfection experiment, with all treatments in quadruplicate. COS-1 cells were transfected with 3.1-RXRα expression vector, the 2B6-XREM-PBREM reporter, the pRL-CMV vector for normalization of transfection efficiency and either CMV2-CAR1 (A and E), CMV2-CAR2 (B and F), CMV2-CAR3 (C and G), or CMV2-PXR (D and H). Panels (A), (B), (C), and (D) show activity of various phthalates with each of the receptors. Panels (E), (F), (G), and (H) show a more complete dose-response curve comparing DEHP, DiNP, and the positive control CITCO for CAR1, CAR2, and CAR3 or TO901317 for PXR. All treatments were for 24 h, and the data are represented as normalized luciferase values and each data point represents the mean (±SEM). *p < 0.05 compared with androstanol control for CAR1 or DMSO control for CAR2, CAR3, and PXR.