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. 2011 Mar 24;2(2):138–145.

Figure 2.

Figure 2.

DNA damage-induced XPA nuclear import is not affected by checkpoint proteins Chk1 and MK2 in the p53-deficient cancer cells. A. H1299 cells were transfected with control siRNA or Chk1 siRNA. After 48 hrs, transfected cells were mock or 20 J/m2 UV-C treated and further incubated for 2 hr for recovery. The subcellular location of XPA was then assessed by fractionation and Western blotting. B. Control siRNA- or MK2 siRNA-transfected H1299 cells were mock-treated or irradiated with 20 J/m2 UV-C followed by a 2 hr recovery. Fractionation and Western blotting were employed to analyze the subcellular localization of XPA and the expression of MK2. C. H1299 cells were pretreated with DMSO, 250 nM UCN-01 or 10 uM SB203580 for 1 hr. Cells then were mock-treated or irradiated with 20 J/m2 UV-C followed by a 2 hr recovery in the presence of the inhibitors. The fractionated samples were analyzed by Western blotting.