Figure 2.

hSERT WT transports DA with a higher maximal velocity than 5-HT. COS-7 cells transfected in 48-well plates with hSERT WT were incubated with 200 nm [³H]5-HT or 200 nm [³H]DA and increasing concentrations of unlabeled 5-HT or DA for 10 min at 37°C, followed by washing and scintillation counting. Mean and SEM from a representative experiment run in triplicate are shown.