Figure 1.
GO-203 disrupts redox balance in CML cells. (A-D) KU812 (A, C) and K562 (B, D) cells were treated with 5 µM GO-203 or 5 µM CP-2 each day for 2 days. The GO-203–treated cells were also incubated concurrently with 5 mM NAC. (A and B) Oxidation of c-H2DCFDA to DCF was determined by flow cytometry. The results are expressed as the relative ROS level (mean ± SD for 3 determinations) as compared to that of control cells (assigned a value of 1). (C and D) Cells were analyzed for GSH levels. The results (mean ± SD of 3 determinations) are expressed as GSH levels/106 cells (µM).