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. 2011 Jan 26;22(6):689–696. doi: 10.1089/hum.2010.130

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Copyright 2011, Mary Ann Liebert, Inc.

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FIG. 1. — Schematic of the lentiviral (LV) vectors and transduction of canine leukocyte adhesion deficiency (CLAD) CD34⁺ cells. (A) pRRLSIN.cPPT.hPGK-cCD18.WPRE was generated by replacing EGFP with the canine CD18 cDNA. pRRLSIN.cPPT.MSCV-cCD18.WPRE was generated by replacing the human phosphoglycerate kinase (hPGK) promoter with the murine stem cell virus (MSCV) promoter/enhancer. (B) Titers of unconcentrated vector stocks were determined by Southern blot analysis as described in the Material and Methods.