Figure 2. Survival and proliferation of CD4+ T cells under Th1, Th2 and Th17 polarizing conditions.
(A) Purified naïve CD4+ T cells were isolated and stimulated as in Figure 1. After 3 days incubation, live and dead CD4+ T cells were counted by trypan blue staining (pooled data of 3 experiments, n = 4 in each group). (B) Naïve CD4+ T cells were labeled with carboxyfluorecein diacetate succinimidyl ester (CFSE) and stimulated for 3 days (representative flow cytometry profile from one of two independent experiments, n=3 in each group).