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. 1999 May;9(5):482–491.

Figure 2.

Figure 2

Figure 2

Allele ratio reproducibility depends on DNA input level. (a) Analysis of input DNA level on allele ratio variability with STR D18S847. Allele ratios represent the average of either nine locus-specific PCR reactions with pooled PEP DNA [three independently derived pools of three PEP reactions, each done in triplicate (dark shaded bars)] or of three independent locus-specific reactions using genomic DNA (light shaded bars). Amount of DNA used in each PEP or genomic locus-specific reaction is indicated on the x-axis. Error bars represent the s.d. of the replicates, and brackets indicate the range of allele ratios for each set of replicates. Values for the s.d. and allele ratio range are given below the graph. Volume constraints prevented the evaluation of genomic DNA at the 3000 cell level. (b) Variability vs. input DNA level into PEP reactions for multiple chromosome 18 STRs. Values indicate the ratio of the variability (s.d.) at a given input DNA level compared with the variability (s.d.) at the highest input DNA level (3000 cells).