Fig. 6. Glucose transporter 4 (GLUT 4) protein expression and localization within the cell.
A) Protein expression of GLUT 4 was significantly increased in the HRCV group, though the HCD group had a trend to increased translation (*P=0.04). B) Immunofluorescence of GLUT 4 in the control group demonstrates diffuse and rather equal distribution of GLUT 4 in the cytoplasm and sarcolemmal membrane. C) GLUT 4 distribution in the HCD group demonstrates areas of high concentration in the cytoplasm. The protein is thought to be inactive in this location. D) In the HCD-R group GLUT 4 is heavily concentrated in the cell membrane. In this location it is active and likely at least partially responsible for improved glucose metabolism (40×, inset 180×, scale bar represents 10μm).
