Table 2.

Sites in myc-Kal7 Phosphorylated in Non-Neuronal Cells

Residue	Score	Peptide Sequence	EN/FT	M/Z	Ion Mass	Charge
487	82.08	K.ALLDVLQRPLpSPGNSESLTATANYSK.A	FT	942.474	2824.399	3
491	59.87	K.ALLDVLQRPLSPGNpSESLTATANYSK.A	EN	942.475	2824.404	3
493/495	56.12	K.ALLDVLQRPLSPGNSEpSLpTATANYSK.A	EN	942.475	2824.404	3
710	28.31	R.pSAPPSLGEPTEAR.D	EN	696.314	1390.614	2
724	47.39	R.SAPPSLGEPTEARDpSAVSNNK.T	EN	1104.002	2205.989	2
963	27.45	K.AEALLQAGHpYDADAIRECAEK.V	FT	1177.522	2353.030	2
1188	22.95	K.LLIQLADpSFVEK.G	EN	728.381	1454.747	2
1249	22.84	K.DLELDIIPASLpSDR.E	EN	818.896	1635.777	2
1520	53.17	K.LLTpSELGVTEHVEGDPCK.F	FT	1003.957	2005.898	2
1590	61.94	K.GALKEPIQLPKpTPAK.L	EN	835.967	1669.919	2
1598	92.54	R.NNpSKRDGVEDGDSQGDGSSQPDTISIASR.T	EN	1024.776	3071.307	3
1608	70.43	K.RDGVEDGDpSQGDGSSQPDTISIASR.T	EN	877.039	2628.096	3
1613	71.06	K.RDGVEDGDSQGDGpSSQPDTISIASR.T	EN	877.039	2628.094	3
1614	57.92	R.DGVEDGDSQGDGSpSQPDTISIASR.T	EN	1334.662	2667.310	2
1618	59.31	R.DGVEDGDSQGDGSSQPDpTISIASR.T	EN	825.007	2471.998	3
1620	65.83	R.DGVEDGDSQGDGSSQPDTIpSIASR.T	EN	825.006	2471.995	3
1625/1626	63.93	R.pTpSQNTVESDKDGNLVPR.W	EN	970.442	1938.870	2
1629	54.98	R.TSQNpTVESDKDGNLVPR.W	EN	970.443	1938.871	2
1632	75.24	R.TSQNTVEpSDKDGNLVPR.W	EN	970.441	1938.867	2
1652	24.06	R.WHLGPGDPFSpTYV.-	EN	778.336	1554.658	2
95-Non	70.54	R.GFTVIIDMR.G	FT	526.284	1050.553	2

The peptides used to identify phosphorylation sites in Myc-Kal7 expressed in stimulated pEAK Rapid cells are identified; formatting for Table 2 is the same as Table 1. Closely spaced Ser and Thr residues, where the phosphorylation site could not be unambiguously assigned, are indicated. Thr⁹⁵ was not identified as a phosphorylation site; data for the non-phosphorylated Thr⁹⁵ peptide (95-Non) are provided. Representative spectra are shown in Supplementary Figs. S23 to S41.