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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Feb 15;254(2):65–71. doi: 10.1016/j.taap.2010.03.023

Fig. 5.

Fig. 5

Cytotoxicity of chronic MnCl2 treatment in differentiated N27 cells. Differentiated N27 cells were treated with 0.1 μM, 0.3 μM or 1 μM MnCl2 for 24 h. The effect of chronic manganese treatment on cell death was quantified by Sytox Green fluorescence assay. The intensity of fluorescence was measured by fluorescence plate reader and shown as % of control. N27 cells exposed to 100 μM H2O2 for 3 h were used as a positive control. The data represent a mean + SEM of four individual measurements. Asterisks (***p<0.001) indicate significant differences between H2O2-treated cells and control cells.