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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Feb 15;254(2):65–71. doi: 10.1016/j.taap.2010.03.023

Fig. 6.

Fig. 6

Effects of PKCδ inhibition on MnCl2-induced reduction of TH activity in differentiated N27 cells. Differentiated N27 cells were treated with 1 μM MnCl2 or cotreated with 3 μM rottlerin for 24 h. A, TH activity: Cells were pretreated with 2 mM NSD-1015 for 1 h before MnCl2 treatment and lysed. Extracts were used for determining L-DOPA levels by HPLC. The data represent a mean + SEM of six to eight individual measurements. Asterisks (**p<0.01) indicate significant differences between MnCl2-treated cells and control cells. B, PKCδ activity: cell extracts from the above treatment were used in the immunoprecipitation kinase assay. Recombinant PKCδ protein was used as a positive control. The bands were quantified by a PhosphoImager after scanning the dried gel and are expressed as a percentage of control. The data represent a mean + SEM of three individual measurements. Asterisks (**p<0.01) indicate significant differences between MnCl2 treated and control cells.