Table 2. Histopathology of Wild Type and Transgenic Mice Receiving Different Doses of DIM for 20 Weeks.
Histopathological Response dichotomized as CIN free or not. For thebinary outcome, CIN freedom, Cochran-Armitage Test for trend, reported significant DIM Dose effect in Transgenic mice (P<0.0001) and not significant in Wild type (P=0.5327). Cochran –Armitage exact test with Hochberg procedure for multiple testing, identified 1000ppm as the minimum effective dose (MED) at (P<0.05)
| Cervical Epithelium | 0ppm DIM | 500ppm DIM | 1000ppm DIM | 1500ppm DIM | 2000ppm DIM | 2500ppm DIM |
|---|---|---|---|---|---|---|
| Transgenic | ||||||
| CIN-free | 3 | 4 | 13 | 14 | 13 | 14 |
| CIN1 | 1 | 3 | 2 | 0 | 0 | 1 |
| CIN2 | 5 | 9 | 4 | 5 | 9 | 5 |
| CIN3 | 6 | 5 | 2 | 3 | 1 | 1 |
| Carcinoma in situ (NCIS) | 2 | 0 | 0 | 0 | 0 | 0 |
| Squamous Cell carcinoma (SCC) | 2 | 1 | 0 | 0 | 0 | 0 |
| Total mice (Transgenic) | 19 | 22 | 21 | 22 | 23 | 21 |
| Percent CIN-free/Total | 16% | 18% | 62% | 64% | 57% | 67% |
| Wildtype | ||||||
| CIN-free | 26 | 27 | 21 | 18 | 19 | 20 |
| CIN1 | 5 | 5 | 0 | 5 | 2 | 4 |
| CIN2 | 3 | 0 | 0 | 2 | 4 | 2 |
| Wildtype Total | 34 | 32 | 21 | 25 | 25 | 26 |
| Percent CIN-free/Total | 76% | 84% | 100% | 72% | 76% | 77% |