Skip to main content
. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Methods. 2011 Jan 20;54(2):284–294. doi: 10.1016/j.ymeth.2010.12.039

Figure 5.

Figure 5

In vitro Dicer processing. Dicer cleavage of 5′-end P32 antisense labeled RNAs [47]. The RNA sense strands (2′-F modified or regular RNA) were annealed with equal molar equivalents of 5′-end P32-labeled complementary antisense RNA strands. The cleavage products or un-cleaved, denatured strands were visualized following 15% denaturing polyacrylamide-gel electrophoresis.