Table 1.
List of identified proteins after “in solution” and “in gel” digestion
| No. | Protein name | Accession No. |
In sol. 75 min. grad1 |
In sol. 4 hr grad2 |
In sol. MS II3 |
In gel4 |
|---|---|---|---|---|---|---|
| 1. | ITI H1 chain | 292530 | + | + | + | + |
| 2. | ITI H2 Chain | 645038 | + | + | + | + |
| 3. | ITI H3 Chain | 876950 | + | + | + | + |
| 4. | Fibronectin | 855785 | + | + | + | + |
| 5. | Heparin Cofactor2 | 879573 | + | + | − | + |
| 6. | Serum albumin | 745872 | + | + | + | + |
| 7. | C4A isoform b | 292950 | + | + | + | + |
| 8. | Alpha 2 HS glycoprotein | 953689 | + | + | + | + |
| 9. | Fibulin 1 | 218803 | + | + | + | + |
| 10. | Vitronectin | 298971 | + | + | + | + |
| 11. | Carboxypeptidase N | 10295 | + | + | − | + |
| 12. | Pregnancy zone protein | 25426 | + | + | − | + |
| 13. | Apolipoprotein A1 | 853525 | + | + | − | + |
| 14. | CD 44 antigen | 955768 | + | + | − | + |
| 15. | HMW Kininogen −1 | 32328 | + | + | − | + |
| 16. | 29 kDa protein | 795830 | + | + | − | + |
| 17. | Complement C3 | 783987 | − | + | + | + |
| 18. | Complement C4B | 654875 | − | + | + | + |
| 19. | Thrombospondin 4 | 328550 | − | + | + | + |
| 20. | Prothrombin | 19568 | − | + | + | + |
| 21. | Gelsolin | 646773 | − | + | + | + |
| 22. | Alpha-2-antiplasmin | 879608 | − | + | + | − |
| 23. | Alpha-2-macroglobulin | 478003 | − | − | + | + |
| 24. | 263 kDa protein | 873210 | − | − | − | + |
| 25. | Hemoglobin | 796636 | − | − | − | + |
| 26. | Clotting Factor IX | 11767596 | − | − | + | + |
| 27. | Actin, cytoplasmic 1 | 21439 | − | − | + | + |
| 28. | Osteonectin | 14572 | − | − | + | + |
| 29. | Apolipoprotein B-100 | 22229 | − | − | + | + |
| 30. | IgG | 884107 | − | − | − | + |
| 31. | HSP 90 alpha, isoform 1 | 784295 | − | − | − | + |
| 32. | HSP 90 alpha Isoform 2 | 382470 | − | − | − | + |
| 33. | HSP 90 beta | 414676 | − | − | − | + |
| 34. | HSP89-alpha-delta-N | 604607 | − | − | − | + |
| 35. | Cartilage oligomeric matrix protein |
28030 | − | − | + | + |
| 36. | Antitrombin III | 32179 | − | − | − | + |
| 37. | Periostin | 218585 | − | − | − | + |
| 38. | TGFBI – Transforming GF beta ind. protein |
292950 | − | − | − | + |
| 39. | ASHG 29 kDa protein | 795830 | − | − | − | + |
| 40. | Vitamin D binding prot. | 555812 | − | − | − | + |
| 41. | Tubulin alpha | 930688 | − | − | − | + |
| 42. | Tubulin beta | 645452 | − | − | − | + |
| 43. | PSMA 7, subunit alpha | 218372 | − | − | − | + |
| 44. | YWHAE 22 kDa protein | 793344 | − | − | − | + |
| 45. | F5 252 kDa protein | 22937 | − | − | − | + |
| 46. | Protein S | 294004 | − | − | − | + |
| 47. | Coagulation factor X | 19576 | − | − | − | + |
| 48. | Apolipoprotein C3 | 28748 | − | − | + | − |
| 49. | Lumican | 51884 | − | − | + | − |
1
“In solution” digestion, 75 min gradient, LC Packings/Sciex-Applied Biosystems LC-MS/MS (see Materials and methods).
2
“In solution” digestion, 4 hrs gradient, LC Packings/Sciex-Applied Biosystems LC-MS/MS (see Materials and methods).
3
“In solution” digestion, Agilent Technologies-Thermo Electron Corporation LC-MS/MS (see Materials and methods).
4
“In gel” digestion, 75 min gradient, LC Packings/Sciex-Applied Biosystems LC-MS/MS (see Materials and methods).