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. 2011 Jun;42(3):360–367. doi: 10.1016/j.nbd.2011.01.029

Fig. 2.

Fig. 2

Analysis of the inter-cellular transfer of alpha synuclein by exosomes, a. Western blot analysis of alpha-synuclein levels (using anti-alpha-synuclein and HA antibodies) relative to actin in normal SH-SY5Y cells following overnight treatment with exosomes isolated from CM from normal and alpha-synuclein over-expressing SH-SY5Y cells. b, Immunofluoresence staining for alpha-synuclein (i and ii, anti-alpha-synuclein antibody; iii and iv, anti-HA antibody, arrows) in normal SH-SY5Y cells (i and iii) and after 16 h exposure to exosomes isolated from CM from alpha-synuclein over-expressing cells (ii and iv). c, Serial confocal Z-projection images (4 μm) of normal SH-SY5Y cells 16 h after incubation with exosomes isolated from WT alpha-synuclein over-expressing cells, anti-alpha-synuclein antibody (green) and DAPI (blue). d. Western blot analysis of alpha-synuclein levels (alpha-synuclein antibody) relative to actin in normal SH-SY5Y cells following overnight treatment with intact and sonicated exosomes isolated from CM from alpha-synuclein over-expressing SH-SY5Y cells. e. Immunofluoresence staining of alpha-synuclein (alpha-synuclein antibody, green) relative to DAPI (blue) in differentiated normal SH-SY5Y cells grown under control conditions (i) or 16 h after treatment with exosomes isolated from CM from alpha-synuclein over-expressing cells (ii).