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A × 2 slugs expressing GFP-DimB and stained with neutral red. GFP-DimB cells were stained with neutral red and allowed to migrate under unidirectional light until about 20 h of development. The ventral surfaces of two slugs (A and B) are visualised in the confocal microscope for both GFP fluorescence and transmitted light. The images were merged and the neutral red stained vesicles can be seen as dark granules, surrounding or flanking at one side the fluorescent nuclei.
