Fig. 9.

ChIP analysis of DimB binding to the ecmB promoter. Cells were incubated with or without DIF for 4 h and subjected to ChIP analysis as described in the Materials and methods section. The absolute recoveries from the procedure varied from experiment to experiment, (three independent experiments with triplicate Q-PCR analysis in each) but the induced signal for ecmA was always the highest. Therefore values are normalised to this and are shown with their Standard Deviations and with the Student's paired T test; applied to the ecmB analysis with and without DIF-1 and in samples immuno-precipitated from GFP-DimB transformant cells. As indicated by the asterisk the induction by DIF is significant with a P < 0.05.