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Changes in tertiary structure of hen egg white lysozyme (20 μM) in 200 mM ammonium acetate (pH 7.4) solutions as a function of DMSO concentration measured by (a) circular dichroism at 289 nm (wavelength scans inset) and (b) solution hydrogen/deuterium exchange mass spectrometry (HDX-MS); (c) the average charge of ions in these HDX-MS experiments calculated as the abundance-weighted average of all the charge states in the distributions
